Agriculture
Mozhgan Esmaeelian; Javad Feizy; Moslem Jahani; Soodabeh Einafshar
Abstract
In the present study, the conventional solvent extraction (CSE) was compared with ultrasound-assisted extraction (UAE) and subcritical water extraction (SWE) methods for the extraction of bioactive constituents from Crocus sativus corms. The response surface methodology (RSM) based on a central composite ...
Read More
In the present study, the conventional solvent extraction (CSE) was compared with ultrasound-assisted extraction (UAE) and subcritical water extraction (SWE) methods for the extraction of bioactive constituents from Crocus sativus corms. The response surface methodology (RSM) based on a central composite face-centred design (CCD) was used to optimize the extraction parameters. The optimum conditions of extraction were estimated to be ethanol 80%, 48˚C and 60 min in CSE, ethanol 80%, 37˚C, 45 min, and 37 kHz in the UAE, and 180˚C and 22 min in SWE. The subcritical water extraction showed the maximum extraction yield, and the highest value of total phenolic compounds (807.6 mg gallic acid equivalent in 100 g saffron corm) and total flavonoid (12.2 mg Quercetin equivalent in 100 g saffron corm) were determined in the SWE extract. The phenolic and flavonoid content of the SWE extract is 8 and 12 times higher than the other two methods, respectively. Also, CSE and SWE extracts showed the least and the most antioxidant activities, respectively. The antibacterial activity of the optimized extracts was evaluated by the microdilution method. The results showed the minimum bactericidal concentrations of 300 and 600 mg mL-1 against Staphylococcus aureus and Escherichia coli bacteria, respectively.
Soodabeh Einafshar; Parvin Sharayei
Abstract
Saffron is a unique product of Iran and Saffron corm is one of the saffron producing ingredients that is produced annually in high volume in the country. It is shown that saffron corm is a low- cost material, contains some bioactive compounds that are extractable. The extraction method is effected on ...
Read More
Saffron is a unique product of Iran and Saffron corm is one of the saffron producing ingredients that is produced annually in high volume in the country. It is shown that saffron corm is a low- cost material, contains some bioactive compounds that are extractable. The extraction method is effected on the kind and amount of bioactive material extracted from saffron corm. Therefore, this research was carried out to produce high value added materials from waste saffron onion. First, the saffron onion was prepared, dried and completely grinding. Extracting was performed using solvent (80% methanol, 80% ethanol and water) and ultrasound (100% intensity, 0, 20 and 40 minutes at room temperature). The extracts were dried at rotary evaporation. In each case, the extraction efficiency, determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), phenolic compounds, iron regeneration strength III, and free radical receptivity were determined. In order to investigate the independent effect (type of solvent, ultrasound intensity) and interaction (solvent type and ultrasound intensity) on bioactive compounds of saffron onion extract, a factorial arrangement of 2 variables with completely randomized design was used in three replications. The mean comparison was done by Duncan method. The results showed that Ethanol solvent extracted 85.84 mg/ml of phenolic compounds with the highest reducing power of FeIII (594.7 μmol/ml) and radical receptivity (59.42%), and the ultrasound process at 100% for 40 minutes extracted the highest amount of phenolic compounds (82.23 mg/ml).
